FGF14 Knockout cell line (H9)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

FGF14 Knockout cell line (H9) Order

Catalog#

YKO-H1005

Size

2*10^5

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FGF14 Knockout cell line (H9)

Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

FGF14

Gene ID

2259

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

Target region

Knockout region: Exon 2 (based on transcript FGF14-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:H9			STR Info (Cell bank) Cell Line:WAe009-A-5
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	9	9		9	9
D7S820	9	11		9	11
D16S539	12	13		12	13
VWA	17	17		17	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	10	11		10	11
CSF1PO	11	11		11	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FGF14 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FGF14 gene knockout FGF14 Knockout cell line (H9) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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